Immobilization of Mutant Phosphotriesterase on Fuller’s Earth Enhanced the Stability of the Enzyme

Immobilization is a method for making an enzyme more robust in the environment, especially terms of its stability and reusability. A mutant phosphotriesterase (YT PTE) isolated from Pseudomonas dimunita has been reported to have high proficiency hydrolyzing Sp Rp-enantiomers organophosphate chromophoric analogs therefore great potential as decontamination agent biosensor. This work aims investigate feasibility using Fuller’s earth (FE) YT PTE immobilization support characterize biochemical features after immobilization. The immobilized was found show improvement thermal with half-life 24 h compared that free enzyme, which only 8 h. allowed storage up 4 months reuse 6 times. showed tolerance against all tested metal ions, Tween 40 80 surfactants inorganic solvents. These findings became use regards would enhance future applicability this other suitable industrial applications.